Review

flagnicd3 (Addgene inc)

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Structured Review

Addgene inc flagnicd3
Flagnicd3, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/3xflagnicd3/3XFlagNICD3+(Plasmid+%2320185)/pmc12977634-283-21-23
Average 93 stars, based on 8 article reviews

flagnicd3 - by Bioz Stars, 2026-09

93/100 stars

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Transfection:

Article Title: HCMV promotes viral reactivation through the coordinated regulation of Notch signaling by UL8 and miR-UL36
Article Snippet: .. HEK293T cells were seeded into 96 well plates and transfected with 50 ng of 4XCSL-Luciferase (41726; Addgene) vector, 50ng 3XFLAGNICD3 (20185; Addgene) and 100 fmol of negative control or miRNA mimic using Lipofectamine 2000 (Invitrogen). .. Twenty-four hours after transfection cells were harvested for luciferase assay using the Dual-Glo Reporter Assay Kit (Promega) according to the manufacturer’s instructions.

Negative Control:

other:

Article Title: Area-Specific Regulation of Quiescent Neural Stem Cells by Notch3 in the Adult Mouse Subependymal Zone
Article Snippet: 3XFlagNICD1 and 3XFlagNICD3 were purchased from Addgene (plasmids #20183 and #20185, respectively) and inserted into pCAG2IG to generate pCAG2IG-NICD1 and pCAG2IG-NICD3.